FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005179732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47078943
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATGGG1294905827.504988801469054No Hit
GTCTCCTT1289955227.399833509431172No Hit
TCACATCA995164721.138212470063316Illumina PCR Primer Index 1 (100% over 8bp)
AGTGGAAC973983920.688312819597503No Hit
AGTGGCAC2088650.44364844809706117No Hit
TCACCTCA1115600.23696368884067764RNA PCR Primer, Index 40 (100% over 8bp)
TCCCATCA816670.1734682106180676No Hit
AGTGGACC644840.1369699400430464No Hit
CAGCTGGG597490.12691236504608866No Hit
GGCTCCTT567480.12053796534896716No Hit
GTCTCCGT515590.10951605264374775No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)