FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005179734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30563225
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC1337583443.764471844839676No Hit
CTAGGTGA866882628.363584013139974RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAATT713141723.333326244203615No Hit
TCGTTCAG1837610.6012487229341799No Hit
GATACGCA1591920.5208612638227804No Hit
CTAGGTGC624650.20437960980884706No Hit
GATACGAC516010.16883362276068709No Hit
GATACTCC478040.15641019558636238No Hit
ATAGGTGA391730.12817037469049813RNA PCR Primer, Index 39 (100% over 8bp)
GATCCGCC381620.1248624776999155No Hit
AGCCAATA368790.12066462227071914No Hit
GATAAGCC361250.1181976051283855No Hit
AGCAAATT357220.1168790270005865No Hit
GATACCCC356500.1166434497668358No Hit
GAAACGCC341890.11186319506531134No Hit
AGACAATT339720.11115319145803494No Hit
GCTACGCC313720.1026462357948155No Hit
CTAGGTAA306210.1001890343705548No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)