FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22564869
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCC639885328.357589844638586No Hit
TTGCCGGA555387424.612923744427675No Hit
AGTTGCAG463614220.545840527591807No Hit
CAAGAATT463121620.524010132742184No Hit
GCCATTCA1564340.6932634973418192No Hit
GCCATTAC743590.329534374872728No Hit
AAAGAATT540960.2397354932572398No Hit
TTGCCGGC455520.20187132484571482No Hit
GCAATTCC447830.19846337242197148No Hit
GACATTCC436290.193349227952531No Hit
GCCCTTCC400900.17766555613506996No Hit
TTGCCTGA396480.1757067590332565No Hit
TTGACGGA374370.16590834185653813No Hit
AGTTGAAG322120.1427528783792186No Hit
CAAGAATA321720.14257561167317212No Hit
GCCATACC319440.14156519144870727No Hit
CAATAATT297850.1319972209898493No Hit
TTGCCGAA290420.12870449192503622No Hit
TTGCAGGA272580.12079839683536385No Hit
CAAGAAAT263560.11680103261401606No Hit
ATGCCGGA242910.1076496389143673No Hit
ATTTGCAG239560.10616503025122814No Hit
AGTTGCAT238750.10580606517148405No Hit
GCCAATCC234350.10385613140497293No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)