FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25623390
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG667570.26053149095416334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA152450.018.04681
TGCCAAA121800.017.5794961
CGGCTAG118000.017.493021
GGGACCT71350.017.422651
AAACCTG105100.017.4134751
GACTAAC85700.017.2243421
CCGGTAG71200.017.0239241
ACGGAGA109050.016.8744351
AGACGTT85050.016.8033161
CAATGGA88100.016.7985928
TCCACGA81500.016.7602378
TACGGAT89950.016.7329461
CTTAACT96350.016.7217181
TATGCCC93450.016.7055451
GATGCTA79150.016.6912541
GTTTGAC86800.016.635458
GATCGCG67450.016.6211361
CGACCTT80900.016.6021581
TTTGACA75450.016.5932799
ACTGAAC73150.016.5154231
Produced by FastQC (version 0.11.5)