FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21722071
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5233732.4094065432342986No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT730440.336266279582642No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340910.15694175753315603No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT244700.11265040059946403No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA227560.10475980858362906No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA219220.10092039566577239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT981150.079.777841
AGCAGTG1008150.077.8112952
CAGTGGT1164450.067.2275544
GCAGTGG1210050.065.4921953
AGTGGTA1291850.061.3131835
TGGTATC1338400.057.58277
GTGGTAT1359650.057.437646
GTATCAA1369950.056.5619629
GGTATCA1371000.056.5089658
ATCAACG1359050.030.01192510-11
CAACGCA1379050.029.7881112-13
ACGCAGA1405250.029.2178714-15
AGAGTAC1418750.028.73366218-19
TCAACGC1357400.028.66899512-13
GCAGAGT1433350.028.53551316-17
AGTACTT1420150.028.20804820-21
AACGCAG1407250.028.02589414-15
CGCAGAG1431050.027.52238816-17
GTACTTT1403700.027.48026822-23
TACTTTT1490350.027.3175522-23
Produced by FastQC (version 0.11.5)