FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16596395
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15550.049.537519
AGTCTAG11200.025.0684459
GTCTAGT15900.016.7527229
CTAGTCC17400.013.32254410-14
TCTAGTC17250.012.102900510-14
CTAGATC16600.011.887156530-34
TAGTCTA29800.010.8711898
TCGTCTA83150.010.2164638
GCTAGAT19300.08.88086230-34
GTCGTCT100850.08.8516987
GGCTAGA25850.06.519147430-34
GGTTAGG44100.06.333920515-19
CGGTTAG31900.06.22870715-19
TGTCGTC167000.06.1644626
GTAGTCT70900.05.6862067
ATTGTCG160650.05.6026044
TCCATTG164350.05.5813351
CCATTGT165200.05.49234442
AGTCACA83650.05.42225360-64
GTCTAGC12750.05.19467310-14
Produced by FastQC (version 0.11.5)