FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13962546
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG41800.041.4675641
TGAGTGA48450.036.560492
GAGTGAT56750.031.5912483
AGTGATG86150.020.9772554
ATGGTTG96400.018.7473268
GATGGTT111450.015.9565617
TGGTTGA116700.015.6096749
TAGTGTG28000.012.69977915-19
GTGATGG193350.09.7560345
TGATGGT196000.09.2199636
GTAGTGT41150.08.88630315-19
CCCTAAG14609.557843E-67.3969036
AGTGTGG55550.06.79007315-19
CTAAGAG18254.358739E-66.7070068
TACCCTA21801.5948845E-66.2751964
ACCCTAA25305.204856E-75.97606045
GGTAGTG72200.05.523314515-19
TGAGGTA83450.05.021250710-14
Produced by FastQC (version 0.11.5)