FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15725607
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGA15800.013.76157915-19
GGACTAG17950.012.03303615-19
TATCCTA54800.011.9557498
CTAGACG17200.011.30189420-24
ATCCTAA64300.010.9731449
ACTAGAC18600.010.29638120-24
CCAGGTA92550.09.5697353
AGGTATC100400.09.1071645
GTATCCT114950.08.3928687
CCTAACC28450.08.3511759
AGCCAGG102950.07.71601961
CAGGTAT115400.07.6742264
CTAACCA29401.8189894E-127.1017477
TCCTAAC36800.06.651928
GCCAGGT137500.06.497142
CTAACCG31100.05.972792610-14
GGTATCC157300.05.8586086
ACTGTAG64000.05.66993540-44
CTAGACT13250.05.54246215-19
ACGTGTA68700.05.302898455-59
Produced by FastQC (version 0.11.5)