FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17394107
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15300.090.3527159
AGTCTAG7200.031.999929
CTAGTCC15350.019.69997210-14
TCTAGTC15450.019.10645310-14
GTCTAGT15550.017.77974510-14
TCGTCTA87300.017.237078
CTAGATC16800.015.17249130-34
GCTAGAT17650.014.36021230-34
CCATTGT100900.014.3510882
TCCATTG111850.012.886691
GGCTAGA19650.012.75203730-34
TAGTCTA17000.012.7058528
TGGCTAG19900.012.08526830-34
GTCGTCT132200.011.3826597
CATTGTC133600.011.3741723
ATTGTCG146100.010.4964414
GGTTAGG28650.010.3533915-19
GCTAATC51750.09.87767570-74
AGTCACA59400.08.55713160-64
CGGTTAG31800.08.1052415-19
Produced by FastQC (version 0.11.5)