FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17394107
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCTA55050.019.3565248
ATCCTAA55900.019.1910489
GGACTAG20000.017.99789815-19
GACTAGA19050.017.9884515-19
ACTAGAC18550.016.9206520-24
CTAGACG19350.016.51872420-24
AGGTATC125600.012.0384385
CCAGGTA135400.011.274673
GTATCCT138000.010.9041087
CAGGTAT149200.010.5214194
AGCCAGG128050.09.7651991
CCTAACC35200.08.3862049
GGTATCC193250.08.3451956
GCCAGGT184800.08.2996172
GACTGTA77500.08.08194740-44
CTAACCG35950.08.01099210-14
TAGAACT97150.06.8773745-49
ACTCTGA80600.06.64598950-54
AACGTGT99500.06.59939655-59
ACTGTAG60650.06.410050440-44
Produced by FastQC (version 0.11.5)