FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15165846
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCTA50750.022.9834168
ATCCTAA51450.022.9508279
CCAGGTA71550.022.8463213
GACTAGA20700.017.7365115-19
GGACTAG20950.017.1812315-19
ACTAGAC19750.016.91295420-24
CTAGACG19950.016.743420-24
AGGTATC100350.016.1437725
GCCAGGT106500.015.9572542
CAGGTAT106100.015.33886054
GTATCCT114950.013.905237
AGCCAGG110400.013.1584351
GGTATCC153250.010.8052736
CTAACCG32350.09.39220910-14
CTAGACT7750.08.17427415-19
CCTAACC35450.08.1242579
GACTGTA85200.07.537470340-44
TAGAACT88150.07.481495445-49
TCCTAAC40850.07.22648958
AACGTGT103750.06.85642755-59
Produced by FastQC (version 0.11.5)