FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17574208
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGAACACGTGTAGATCT186980.1063945527445675Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA193400.016.040871
TCGGAAG213350.014.84520153
ATCGGAA254100.012.5509272
AGAGCGT249950.012.150778
GTGTTAG56150.010.61318125-29
AAGAGCG291950.010.5014217
GAGCGTC314250.09.8478779
ACACGTG63450.09.37070535-39
CACGTGT64700.09.25641735-39
GGAAGAG352600.09.1037815
TGTTAGA66750.09.07843530-34
TTAGAAC68950.08.74701630-34
GAAGAGC368500.08.5937596
CGGAAGA370900.08.5784014
GTTAGAA72200.08.51276930-34
AGTGTTA75550.07.98315525-29
ACGTGTA79200.07.725339435-39
CACGCTA25805.456968E-127.53166537
AACACGT94250.06.873611535-39
TAGATCT93800.06.84572240-44
Produced by FastQC (version 0.11.5)