FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16041143
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACTC66550.023.582358
CCACTCA79700.019.781499
AAGCCAC108750.014.8283216
GTTATGT102000.013.4320541
AGCCACT126350.012.591877
TATGTTA141600.010.066583
ACAAAGC166500.09.9015063
GTTAAAT155050.09.0539086
TGTTAAA175900.08.226515
ATGTTAA185650.07.7944694
TTATGTT184350.07.69341852
CTCACTA47800.07.409682815-19
GGCCTAA52450.06.973190375-79
TACACAC51250.06.940024450-54
AAAGCCA250450.06.9275785
GCCCCTA52800.06.9006763110-114
TACAAAG251100.06.85247472
ATACAAA271000.06.79430771
TAGGGGC51900.06.5756326105-109
CAAAGCC290950.06.16121534
Produced by FastQC (version 0.11.5)