FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16707268
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAT101500.010.051341
GGCCCTA21850.09.8834684
TCCCCAT127600.08.6313547
TCCCATC128800.07.9371632
ATCCCCA149450.07.89925586
CCCCATC159850.07.070018
CCCATCC153150.07.0032939
CGTCTAA25251.1061275E-86.55701163
CCATCCC160200.06.2907884
CCTAATA40300.05.86006525-29
CTAATAG40450.05.838334625-29
GTTCCTA39552.3646862E-115.8250052
TACTAAC22802.1927699E-55.6829944
CATCCCC214900.05.59396275
CCCTATT51050.05.4992966
CGACTAA17200.00404294635.44069533
TCCTAAT49500.05.38182620-24
TACATAC73600.05.2814627
CCTCACT53600.05.23760610-14
ACATACC79700.05.0578128
Produced by FastQC (version 0.11.5)