FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16707268
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAT104600.010.6016851
TCCCCAT125750.09.2729617
TCCCATC119900.09.1288042
GGCCCTA23203.6379788E-128.0667154
CCCATCC153000.07.9507249
ATCCCCA156750.07.6227556
CCCCATC159350.07.54355058
CCATCCC166800.07.1203164
CTAATAG38800.06.642098425-29
CGACTAA16907.389428E-56.38892273
CCTAATA40100.06.21134625-29
CATCCCC228050.05.99701175
GTAGCTA27858.179268E-85.9469212
TCCTAAT50400.05.512794520-24
CCCTATT51600.05.44034346
GATCTAA18550.0016627145.43259333
GAGCTAA24356.2507985E-55.3210413
CCTCACT56550.05.295084510-14
TGGGGGG53150.05.1737638105-109
ACATACC78700.05.12182478
Produced by FastQC (version 0.11.5)