FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17925323
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT236220.13178005216419253TruSeq Adapter, Index 1 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA203600.018.8837099
AGAGCAC209650.018.3731128
GATCGGA260100.015.179791
TCGGAAG296100.013.4802193
AAGAGCA309550.012.7692497
ATCGGAA343250.011.8804112
GAAGAGC349250.011.4414336
GGAAGAG383000.010.5830395
CGGAAGA428450.09.3261444
AGTCACT93550.08.61942825-29
CTCGCTA43300.08.3139227
CAGTCAC112650.07.59258125-29
CTCGTAT115750.06.991172340-44
TCTCGTA130300.06.45361640-44
CCAGTCA129650.06.41932725-29
GGGGTCT65300.06.2890822
GTCACTT136500.06.202672525-29
TCGTATG134400.06.1389245-49
CACACGT138200.05.907716310-14
ACGCAAT146700.05.88922335-39
Produced by FastQC (version 0.11.5)