FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14241878
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAACACTATAGCCCACATACTATATAGCTCAATAGCTCTACTACT159420.1119374846491453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA105800.025.6470439
ACACTAT115900.023.4741558
AAACACT116250.023.2154566
AACACTA119900.022.4494177
AAAACAC140400.019.5810815
ACAAAAC148600.018.4078393
CAAAACA161850.017.0329174
ATACAAA267200.010.8043721
CTATAGC55300.010.22936910-14
TACAAAA284350.09.9512232
ACACACT58400.09.61080450-54
TACACAC59250.09.59437450-54
CTACACA65700.08.69636845-49
CCGTTAA72950.08.5841434
GAGCCCC67050.08.435263130-134
AGCCCAC78050.07.92889415-19
AGAGCCC72050.07.789964130-134
CGTTAAA88350.07.7385135
GGCCTAA75950.07.69341275-79
CACACTT76850.07.67799650-54
Produced by FastQC (version 0.11.5)