FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17509509
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCACGTTGTGTAGATCT255920.1461605805165639Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA197200.020.8729171
AGAGCGT254650.016.5326758
GAGCGTC256050.016.245729
TCGGAAG274100.015.2056833
AAGAGCG288900.014.3734327
ATCGGAA298000.014.0116832
GAAGAGC358850.011.8532586
GGAAGAG368700.011.53675655
CGGAAGA410650.010.3076414
TAGATCT85800.09.88388440-44
GTAGATC109400.08.14653640-44
AGATCTC114250.07.776633745-49
TGTGTAG121050.07.23166740-44
GTAGGGA123900.07.14417915-19
GAGTGTA129750.06.955754325-29
TAGGGAA136100.06.62010315-19
AGAGTGT135550.06.6156525-29
TGTAGGG137550.06.550316315-19
GTTGTGT148650.06.17884135-39
GTGTAGA150400.06.174638340-44
Produced by FastQC (version 0.11.5)