FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14208860
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC340060.2393295450866572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA116700.014.5563419
AGAGCAC125450.013.5984298
GATCGGA151700.011.7012481
TCGGAAG199100.08.9684073
AAGAGCA197150.08.8354697
ATCGGAA220900.08.5722512
GAAGAGC244150.07.28201066
GGAAGAG255500.06.98699055
AGTCACA51350.06.448222625-29
TACACTA29657.517883E-96.06912958
CGGAAGA327100.06.00774534
TGCCTAA54800.05.80610935-39
CAGTCAC62150.05.5361725-29
GCCTAAA59500.05.516852435-39
CCTAAAT62350.05.403222635-39
CTACACT32252.7674105E-75.3566427
CTAAATC68400.05.156837535-39
TCACATG63350.05.1133230-34
CTCGTAT71400.05.06120840-44
Produced by FastQC (version 0.11.5)