FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15815831
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA17101.9460458E-77.5796643
GACTCTA23602.6975613E-97.0181012
CGTCTAA18557.554863E-76.9871843
TACGTAA27752.4374458E-106.74658443
GTACGTA29351.2914825E-106.62459662
ACTCTAA22502.7329297E-66.08057553
CGTACGT39601.07320375E-105.7068971
GATCTAA18200.0078177245.1433443
TAGGCTA19800.00374518735.0917452
GTTCCTA37151.8337414E-75.03985832
Produced by FastQC (version 0.11.5)