FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17794802
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA20400.010.5907223
GATCTAA11101.8687751E-79.7320153
TAGGCTA21653.5070116E-97.32109932
GTAGCTA24706.890332E-96.7087622
CTCCTAA31701.2369128E-106.3611153
GTCTAAG16604.0155055E-46.07371574
GTCTCAG39803.6379788E-126.03107641
CGACTAA17709.1231684E-45.6962533
TTAGGCT29155.869948E-65.24016141
ATCTAAC19700.00351321815.1179534
AGGCTAA35904.684298E-75.01510523
Produced by FastQC (version 0.11.5)