FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005179997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005179997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16870298
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT186800.11072714898100791Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA281600.011.2872521
AGAGCGT271950.011.1724198
GAGCGTC303050.010.2634489
AAGAGCG312300.010.0054747
TCGGAAG322750.09.7488443
ATCGGAA363700.08.9504012
GAAGAGC373650.08.7094776
GGAAGAG395150.08.3267245
TAGATCT86850.07.72619340-44
CGGAAGA476400.07.01259954
AGTGTAG98000.06.626739540-44
CAGTGTA105400.06.40779235-39
AGATCTC114200.05.96405545-49
TCAGTGT117450.05.836199335-39
GTAGATC118450.05.77441540-44
GTGTAGA129600.05.63316440-44
AGAGTGT124900.05.268846525-29
GAGTGTT125450.05.26870425-29
CGTATCA142300.05.150653460-64
CTTCAGT139300.05.106837735-39
Produced by FastQC (version 0.11.5)