FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17162605
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC192390.1120983673515763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA14550.015.8355663
GAGCACA175250.012.2414829
AGAGCAC187400.011.7551358
CGTCTAA27500.011.2585483
GATCGGA202800.010.8541581
AAGAGCA251700.08.8951437
TCGGAAG259350.08.7729863
GACTCTA26700.08.3602362
ATCGGAA281700.08.3585142
TAGGCTA27850.08.015022
GAGCTAA33700.07.90530163
CGACTAA21503.8198777E-107.70257573
AGATCTA36800.07.4353932
GGAAGAG306450.07.3528885
GAAGAGC316750.07.2956116
ACTCTAA32550.07.07857133
CCTAGGC10300.00572265456.9893375
AGTGTAA50100.06.4672763
TAGAGTA31951.4915713E-106.3103772
CTACTAA46100.06.24749143
Produced by FastQC (version 0.11.5)