FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17162605
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG205160.11953896276235454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA15000.014.4047123
GATCGGA204650.010.7696431
AGAGCGT224850.09.7666328
CGTCTAA26750.09.1543963
TCGGAAG258850.08.5421033
GAGCGTC258900.08.5377659
AAGAGCG273400.08.4273187
GACTCTA27650.08.0780112
ATCGGAA283900.07.89283232
ACTCTAA29000.07.69906953
TAGATCT63000.07.42787540-44
ACACTAT46250.07.31684738
GGAAGAG320050.07.19879675
GAAGAGC329300.07.0842066
AACACTA43300.06.983927
AGATCTA37600.06.89846662
CGACTAA21953.5344783E-86.8906373
CACTATA48900.06.47860779
CGGAAGA387300.06.02520854
TAGGCTA29303.643254E-85.9017592
Produced by FastQC (version 0.11.5)