FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13410302
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA123950.09.3516679
GATCGGA136000.09.0815861
TCGGAAG139600.08.8328863
AGAGCAC142150.08.3062828
ATCGGAA167950.07.7283082
ATCACAC59450.07.6295418
GAATCAC67150.07.07632166
TCACACC72750.06.53161579
AAGAGCA199250.06.3233827
AATCACA69750.06.2964447
TGAATCA76000.05.96810775
ATGAATC87950.05.8120884
CCACTAA22300.05.682255320-24
GGAAGAG231700.05.5620735
AGTCACA42050.05.51364925-29
GAAGAGC253600.05.4224286
CGGAAGA243750.05.37571764
Produced by FastQC (version 0.11.5)