FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14492174
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC192970.1331546253860877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA45150.018.8157449
GCCACTC78200.011.9683368
AGCCACT89800.010.0214577
AAGCCAC96250.09.7995626
GGCCTAA19350.09.22725175-79
GAGCACA150400.08.8077949
TACAAAG126250.08.2143012
AGAGCAC171750.07.58715538
CCCTTAA47800.07.53157334
ACAAAGC131450.07.12233353
ATACAAA156150.06.63091751
CTCACTA30000.06.191581215-19
GTCCCTA32700.06.1654315110-114
GATCGGA230150.05.8516931
TCGGAAG235350.05.69165133
AAAGCCA182950.05.6279115
AAGAGCA251600.05.5798327
ATCGGAA264800.05.4394072
TCCCTTA75700.05.2324653
CCTTAAA82700.05.136775
Produced by FastQC (version 0.11.5)