FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14492174
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA46750.022.021259
GCCACTC79100.013.2880858
AGCCACT92150.011.8750187
AAGCCAC100250.010.8437276
GGCCTAA21000.09.94247375-79
ACAAAGC132500.08.8628643
CCCTTAA44850.08.8349194
TACAAAG128350.08.8147732
ATACAAA165500.07.29910661
CTCACTA32200.06.931069415-19
AAAGCCA186350.06.45339875
AGAGCGT215050.06.26028
GTCCCTA35100.06.112913110-114
TCGGAAG230750.05.80729063
GATCGGA233550.05.7917921
ACTCACT41000.05.724376715-19
CCTTAAA82350.05.68396235
GGTCCCT43500.05.561464110-114
CTTAAAT81350.05.4868136
TTAGATC22350.05.476125140-144
Produced by FastQC (version 0.11.5)