FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15194890
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA42000.026.3954417
ACACTAT43200.025.4958488
CACTATA48750.022.1500939
AAACACT85200.013.3498076
ACCAAAC104200.010.7071953
CCAAACA116500.09.8250754
CAAACAC118700.09.582175
TACCAAA139650.08.6113872
ATACCAA160650.07.60496661
TATTTAG55750.06.84368329
CTTAAGG38750.06.53962840-44
CCTTAAG38700.06.213232540-44
GTACTAA39850.06.14209230-34
GCCCTTA64750.06.11487444
ACTATAG40350.06.030030710-14
CTATAGC38850.06.00344210-14
TACACTC46000.05.5717874115-119
ACGTATA44800.05.4964676140-144
TTAGGCA49600.05.225433360-64
CCCTTAT82450.05.15132335
Produced by FastQC (version 0.11.5)