FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15116334
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA149500.013.8347711
TCGGAAG168300.012.1080123
AGAGCGT189800.010.8494768
GAGCGTC204750.010.0563949
ATCGGAA209050.09.9544712
AAGAGCG212700.09.7829377
TAGCGTG47650.08.91434135-39
GAAGAGC240800.08.7011256
TAGCGTA49150.08.46689730-34
GGAAGAG281200.07.68147955
CGGAAGA315650.07.09403854
AGTGTAG61700.06.86149525-29
TAGATCT63600.06.791946440-44
GAGTGTA66750.06.49339525-29
AGCGTAG80200.05.53001530-34
AGAGTGT78800.05.482158725-29
GTAGGGA80650.05.481054315-19
TAGGGAA82650.05.313578615-19
TGTAGGG88800.05.026652315-19
Produced by FastQC (version 0.11.5)