FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14051102
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCC33500.025.1426338
TTAGACC33550.024.460927
GTTAGAC40700.020.1637296
AGACCCC57400.014.7990969
GTAACAC56900.013.790337
TAGTTAG67450.013.341244
GTCTCAG45000.011.3844891
CTCAGTA48500.011.1311443
AGTTAGA77850.011.0039545
TCTCAGT50000.010.3701182
GACTCTA28800.08.148462135-139
TAACACT99950.08.0668678
ATGTAGT122500.08.0653951
TAGACTC31700.07.2667675135-139
AACACTA130550.06.39655549
GTTTTAG115450.06.2758021
TGTAGTT159750.06.17590952
ACACTAG39650.06.172097770-74
GTAGTTA153500.06.0961443
AGTAACA211450.05.99191246
Produced by FastQC (version 0.11.5)