FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533228
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT35000.013.3767421
GTGAGTA35800.012.4709042
TAACCCC39850.011.0193969
ATAACCC73800.06.53550628
GAGTATA84850.06.2783014
GTATAAC85150.06.0025116
TGAGTAT90800.05.39069753
Produced by FastQC (version 0.11.5)