FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12201051
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC42950.018.4434362
ATACGTA60850.014.5149621
GTACACA53250.014.3324435
ACGTACA57500.014.1521073
AGTGAGT35150.013.7995881
ACACATA58400.013.3141327
GTGAGTA37350.012.9180362
TACACAT62350.012.8179036
CGTACAC63550.012.6892644
TACGTTA44950.011.5348863
GTTACGT44950.011.435241
CACATAC76000.010.89397058
TAACCCC43650.010.8858329
ACGTTAA46900.010.74630454
ACATACC77450.010.318199
CCTAGAC15800.010.024931115-119
CTAGACC17100.09.683657120-124
ATCCTAG21400.08.276318115-119
GGTCCTA22400.07.13530640-44
CCTAAGG26300.06.89838545-49
Produced by FastQC (version 0.11.5)