FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18278770
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA200000.010.4735189
AGAGCAC220000.09.4559418
GATCGGA272150.07.94079831
TCGGAAG284600.07.66516923
AAGAGCA304200.07.2172257
ATCGGAA321500.06.71818452
GAAGAGC368600.06.1906126
GGAAGAG365950.06.176435
AGTCACA75700.05.81955725-29
CGGAAGA413200.05.57467174
CTTAATC87200.05.547397635-39
GCTTAAT83800.05.497591535-39
CTCGTAT89050.05.33652740-44
CGCTTAA83150.05.315481735-39
Produced by FastQC (version 0.11.5)