FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18282640
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA179950.015.1642129
AGAGCAC182750.014.8530788
GATCGGA188100.014.8516751
ACTAAAC55500.012.9729733
AACACTA55300.012.4990957
CTAAACA59450.011.6265764
TCGGAAG270200.010.7653593
CACTATA71350.010.4947449
AAGAGCA277350.010.17631157
ATCGGAA297200.09.9084792
ACACTAT66250.09.8898118
TAAACAC80950.09.161215
GAAGAGC329750.08.7775586
ATACTAA82100.08.5066991
GGAAGAG383550.07.77160745
TACTAAA109800.07.01639372
CGGAAGA437300.06.9316264
AAACACT117150.06.57618436
GGGGGGG44250.05.85762742
CACAGTA112200.05.313369330-34
Produced by FastQC (version 0.11.5)