FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18282640
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA189800.014.41517451
AGAGCGT220100.012.3652898
GAGCGTC238800.011.4874369
AAGAGCG243950.011.2154137
AACACTA56800.010.1408457
ACTAAAC54450.010.0495873
TCGGAAG275650.09.9778713
GTCTAGT55300.09.73887830-34
ATCGGAA304350.09.4391322
CTAAACA59500.09.4386564
CTAGTAC60450.09.02828930-34
TGTCTAG59750.09.013556530-34
ACACTAT66050.08.8296758
TCTAGTA69100.08.1690330-34
GAAGAGC340450.08.1633136
CACTATA74700.07.71084369
GGAAGAG388250.07.39935645
ATACTAA79400.07.1637281
TAGATCT82300.06.963791440-44
TAAACAC82400.06.9029135
Produced by FastQC (version 0.11.5)