FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15965140
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA50200.023.8016099
GCCACTC63650.018.8848958
CCGTTAA61900.015.8143094
AGCCACT78800.015.52813157
CGTTAAA80400.014.2345275
TAGGAGG52950.012.5059987
AAGCCAC102350.012.4474946
AGTGAGT42350.011.7570161
GTGAGTA46250.011.6783842
TCCGTTA90700.010.8780733
GTCTCTT81050.09.7569111
TGAGTAG63650.08.48597053
ACAAAGC167050.08.2342783
GTTAAAT142150.08.2028476
CTAGCTC23450.08.10336135-39
GAGTAGG66450.07.90732964
GTTCCGT134450.07.8422971
TACAAAG186650.07.4080942
ATCTAGC36800.07.31522935-39
AGGAGGG100700.07.2191898
Produced by FastQC (version 0.11.5)