FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15965140
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA45750.020.2990389
GCCACTC60550.015.4574318
CCGTTAA64350.014.0986144
AGCCACT71900.013.116217
CGTTAAA83550.012.58019355
TAGGAGG55250.010.4237417
TCCGTTA93900.09.8150993
AAGCCAC100300.09.6895036
AGTGAGT42500.09.1002081
GTGAGTA46550.08.9735322
GTCTCTT81500.08.7747121
GTTAAAT147100.07.4387436
GTTCCGT140200.07.0787291
TGAGTAG62250.06.8243763
ACAAAGC166700.06.5221633
GAGTAGG68650.06.29310664
CTAGCTC26050.06.24587935-39
AGGAGGG105000.06.239658
GGAGGGG113400.05.96748929
ATCTAGC37250.05.914091635-39
Produced by FastQC (version 0.11.5)