FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15623310
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT330770.21171569917002223TruSeq Adapter, Index 1 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA199350.027.2697561
GAGCACA250750.021.1263059
AGAGCAC253400.020.962188
TCGGAAG260750.020.771443
ATCGGAA299000.018.6218012
CGGAAGA363800.014.8974514
AAGAGCA412400.013.3863877
GGAAGAG440350.012.7163155
GAAGAGC493400.011.276316
AGTCACT99150.011.0921425-29
CAGTCAC106800.010.31109725-29
GTCACTT109050.010.05874925-29
ACGCAAT119250.09.46454235-39
CACGCAA139500.08.11129735-39
CTCGTAT136200.08.0547440-44
CACACGT138100.07.98452310-14
CACGTCT140250.07.95449710-14
CCAGTCA143650.07.896511625-29
ACACGTC143300.07.84546510-14
ACGTCTG156050.07.121528615-19
Produced by FastQC (version 0.11.5)