FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14847161
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA162950.015.6370889
AGAGCAC170650.015.0158758
GATCGGA194650.013.1295061
TCGGAAG269200.010.058743
ATCGGAA294500.09.4642072
AAGAGCA283750.09.30973157
GAAGAGC279850.09.2594276
GGAAGAG335000.08.0571825
CGCGAGT71650.07.614840530-34
CGAGTAA68700.07.397396635-39
GGGGGGT26601.0913936E-117.3103932
CGGAAGA373550.07.3027634
ACGCGAG77200.06.89957330-34
AGTCACG86050.06.4575525-29
CACGCGA83750.06.32558730-34
GCGAGTA87250.06.319331630-34
CTCGTAT86300.06.289456440-44
TCTCGTA88350.06.241296340-44
AGGGGGG37051.0004442E-105.9293951
CAGTCAC101350.05.638948425-29
Produced by FastQC (version 0.11.5)