FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15954797
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC755970.4738198800022338No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG322740.20228399020056476No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA210480.13192270638103387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA153000.08.9391929
AGAGCAC164400.08.14417658
GATCGGA185450.07.18717341
TCGGAAG251950.05.71519043
GGGGGGG55400.05.4582642
AAGAGCA261100.05.37605677
ATCGGAA275500.05.305052
GAAGAGC286050.05.03296766
Produced by FastQC (version 0.11.5)