FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15954797
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG545440.34186583508395624No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA379960.23814781222224266No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA237440.14882044566283106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA193350.06.86646031
AGAGCGT203300.06.65600978
GAGCGTC216650.06.51164779
AAGAGCG232350.05.7928527
TCGGAAG259700.05.29499053
ATCGGAA279100.05.1095752
Produced by FastQC (version 0.11.5)