FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12805269
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA196700.010.6445181
AGAGCGT198600.010.5854818
GAGCGTC226000.09.397429
TCGGAAG234600.09.14663
AAGAGCG230600.09.1152327
ATCGGAA250350.08.9184082
TAGATCT52950.08.10568540-44
GAAGAGC286250.07.49416836
GGAAGAG299150.07.2677135
TGTGTAG62800.07.109539540-44
CGGAAGA336000.06.57913544
GTGTGTA69750.06.25519935-39
AGATCTC74550.06.240056545-49
GTAGATC78050.05.757330440-44
GGTGTGT80800.05.47103835-39
CTAGATC14600.05.4238553140-144
TAACCCC37557.70342E-95.3683799
GAGTGTA83500.05.327766425-29
AGTGTAC84750.05.266173425-29
TACAGTG82500.05.16551930-34
Produced by FastQC (version 0.11.5)