FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13090558
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT26400.015.2530761
GTGAGTA32050.012.5843992
TAACCCC34050.010.9921679
TGAGTAT67350.06.73719023
ATAACCC64250.06.49751048
GAGTATA68800.06.4863494
GTATAAC68900.05.85006866
TATAACC78550.05.68116767
Produced by FastQC (version 0.11.5)