FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16392266
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGATGTTTGTGTAGATCT288610.17606473687042412Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA225100.021.5522821
AGAGCGT258000.018.8344428
GAGCGTC292050.016.6878439
AAGAGCG298450.016.426477
TCGGAAG305600.016.161313
ATCGGAA319250.015.7634792
GAAGAGC369500.013.4821886
GGAAGAG396600.012.579095
CGGAAGA446900.011.2286684
TAGATCT100150.09.84972640-44
TGTGTAG121050.08.17290640-44
AGATCTC125400.08.06058145-49
GTAGATC129850.07.88519140-44
AGTGTCG129800.07.698578425-29
GAGTGTC135850.07.5359125-29
AGAGTGT145600.07.070830325-29
GTAGGGA155100.06.693331215-19
GTGTAGA158750.06.622068440-44
TGTAGGG162100.06.35099715-19
TCTCGGT175600.06.12523745-49
Produced by FastQC (version 0.11.5)