FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12328286
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT43850.014.4970841
GTGAGTA42900.014.4417232
TAACCCC44750.013.3513859
CACATTA71000.010.6456529
ACAATCC95250.07.865863
CAATCCA98800.07.50437454
ATAACCC78350.07.441968
CCACATT112200.07.1214998
ATCCACA122050.06.42880156
AGCATGA131100.06.3144639
AATCCAC128800.06.2595295
TGAGTAT112300.06.158423
TACAATC128900.06.09188132
CCCTTAA49000.05.8762214
GTATAAC112100.05.7151246
ATCTAGC25900.05.503202425-29
CAGCATG156800.05.4172298
GATCGGA146600.05.3924537140-144
GAGTATA120500.05.37637664
TAGATCG54000.05.306491140-144
Produced by FastQC (version 0.11.5)