FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16463525
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTTGATGGTGTAGATCT195200.11856513110041744Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA201550.016.509751
TCGGAAG233450.014.2478713
AGAGCGT229850.014.1567288
ATCGGAA266250.012.8441672
AAGAGCG262300.012.5700637
GAGCGTC288100.011.5693259
CACTATA53850.010.9622189
ACACTAT57200.010.6977368
GGAAGAG323250.010.4226365
GAAGAGC325750.010.4089466
AACACTA56850.010.3837377
CGGAAGA350600.09.6718814
GAGTGTA91950.07.500958425-29
AGTGTAC94100.07.28367125-29
TAGATCT100400.07.071119340-44
AAACACT96950.06.4601376
AGAGTGT112800.06.344249725-29
GTAGGGA114200.06.178052415-19
GTGTACT118150.06.056972525-29
GTAGATC123700.06.05352840-44
Produced by FastQC (version 0.11.5)