FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14587051
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA56750.018.0147959
GCCACTC70000.014.6047018
AGTGAGT40750.014.5759871
ACGTTAA39650.013.6185964
GTGAGTA44850.013.4851712
AGCCACT82950.013.1051537
TAGCATG41750.012.2434638
TACGTTA48050.011.5376173
AAGCCAC96400.010.9780786
GTTACGT55050.010.3900561
CGTTAAA55300.010.1541855
GGCCTAA23400.08.73818575-79
TAACCCC74450.08.5099089
ACAAAGC143950.07.50236753
TACAAAG162350.07.0958972
TTACGTT93450.06.70315932
GATCGGA180100.06.51458641
ATAACCC112800.06.31871138
GTATAAC98300.06.29841046
GTTAAAT98700.06.1999446
Produced by FastQC (version 0.11.5)