FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16376592
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA138700.014.4784839
AGAGCAC146350.013.7708468
GATCGGA161950.013.0731061
TCGGAAG193500.011.0907293
ATCGGAA227350.09.725622
AAGAGCA223650.09.6226867
GGAAGAG287800.07.7527715
GAAGAGC285050.07.49944976
CGGAAGA322250.06.92396454
AGTCACG74450.05.72333525-29
CACACGT80750.05.57988210-14
CAGTCAC85400.05.343472525-29
CCAGTCA89400.05.29761725-29
CTCGTAT84850.05.12437240-44
CACGTCT92800.05.11904810-14
Produced by FastQC (version 0.11.5)