FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16839235
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA18100.015.5096357
CACTATA34850.010.5337499
ACACTAT36900.010.143618
GAGCACA158700.010.0691229
GATCGGA187600.09.15899851
AGAGCAC186000.08.9395288
TCGGAAG185550.08.8580063
CTAGCTA7100.08.516314525-29
ATCGGAA217650.08.24840452
ACACACT36350.08.1188596
AAGAGCA256050.06.8593137
TATGGGG62700.06.20852333
AGTCACA51850.06.025203725-29
GGAAGAG303800.05.68642145
ATACAAC80450.05.5676091
AACACAC59500.05.4439125
GTATGGG82650.05.40851552
TCTAGCT10700.05.38191725-29
ATCTAGC11100.05.31767325-29
CAACACA66000.05.0168314
Produced by FastQC (version 0.11.5)