FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16839235
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA19850.016.3204697
ACACTAT34800.011.7926768
CACTATA37000.011.4806669
TATGGGG61450.09.4901583
GATCGGA197150.08.7757051
TCGGAAG190400.08.5457823
CTAGCTA8300.07.9801425-29
ACACACT40000.07.9191256
ATCGGAA227900.07.58215572
TAGTGTA40700.07.57068535-39
GGGGCCC84700.07.56468966
GGGCCCC84200.07.2675417
ATCTAGC10400.06.92257125-29
GTATGGG85700.06.8890422
AGTGTAG48500.06.857676540-44
AGAGCGT237550.06.7284458
GGTATGG119450.06.26009371
ATAGTGT55600.06.007987535-39
AAGAGCG281800.05.97799447
TGGGGCC100200.05.9632365
Produced by FastQC (version 0.11.5)